Fulorosenti wamamamayikirosikopu: zimaonetsa kafukufuku

Mawu akuti "maso" ali mizu Greek. Pomasulira kumatanthauza kuphunzira zinthu ndi zida mkulu-mwatsatanetsatane. . M'zaka zaposachedwapa kwambiri otchuka fulorosenti ndi elekitironi wamamamayikirosikopu.

kusamvana

Pansi, monga ulamuliro, kumvetsa mtunda osachepera pa zomwe zingakhale bwino distinguishable zinthu. Pa chigamulocho mphamvu zida mlingo wa malowedwe kudzadalira mu dziko tosaoneka ndi maso, kuwona kukula kwa mchitidwe wa. Ndi kuwonjezeka yaikulu ku maofesi malire akhoza kuphatikiza. Choncho, pali malire, kupitirira zomwe njira za nyengo ndi tanthauzo.

The njira ya kuwala wamamamayikirosikopu: limagwirira

Pamene mphamvu kutembenuka zimene zimatenga thunthu mu cheza kuonekera amapezeka chowala. Njirayi imatchedwa luminescence. chodabwitsa Ichi ndi chifukwa chakuti ena zinthu mchikakamizo cha kuwala kuyamba zimatulutsa kuwala kwa ena (zambiri lalikulu) timaganiza. Komanso, ena zinthu zimene zinthu zachilendo kuyatsa mwachindunji mtundu mchikakamizo cha kuwala ultraviolet amasintha mtundu wake.

kulunjika

Chinthu kuti sangathe kuona pansi UV kuwala, akhoza zimatulutsa luster owala, ngati mankhwala ndi chinthu chapadera. Mu zinthu izo kwa mitundu yosiyanasiyana ya kuwala mu mdima. chowala mwamphamvu ndi osiyana, koma monga ulamuliro, ndi ang'ono. Pa nkhani imeneyi, kuwala wamamamayikirosikopu othandiza m'chipinda chamdima. Pamene ntchito mtundu uwu wa kufufuza angathe kuona kuwala kwa chinthu, chimene chimachokera. Mipweya ya zimakhala, maselo zidzakhudza khalidwe la kuphunzira. Kuwala wamamamayikirosikopu imatengedwa kuti ndi ena mmene phunziro histochemical.

Gawo

может быть первичной или вторичной. Kuwala wamamamayikirosikopu akhoza kukhala yaikulu kapena sekondale. Zikatero, chinthu kukonzedwa ndi mankhwala wapadera kupereka luminescence. основывается на собственной способности исследуемого элемента излучать свет. Pulayimale fulorosenti wamamamayikirosikopu zochokera luso lawo la mchitidwe kuti zimatulutsa kuwala.

zida

Kuwala wamamamayikirosikopu imagwiridwa ntchito zosiyanasiyana zipangizo. The chounikira amagwira ntchito monga maziko a mchitidwe wa. Izo zili ndi UV nyale. Komanso chipangizo amagwiritsa ya Zosefera. Mu ena zipangizo angapo masanjidwe osiyana. Malinga amene mtundu ntchito luminescence malemeredwe - ultraviolet kapena buluu, pakati pa kuwala gwero ndi chinthu pansi kufufuza aikidwa ndi fyuluta yoyenera. Popeza luminescence tosaoneka mchitidwe ankafalitsa mwakhama ofooka malemeredwe opepuka, tidzakwatulidwa yekha pa chikhalidwe chimodzi. Zotsala cheza anakafika ku gwero akalangidwe lachikasu wobiriwira fyuluta. Izo aikidwa pa chida eyepiece. Kwambiri kutchulidwa zotsatira luminescence zidzachitika pamene sefa kwathunthu kudula cheza cha kuwala akutuluka kuchokera ku gwero.

ndi unsembe m'kuunika kumaoneka chiyani? Mmenemo ndi kuwala gwero ndi maso kwachilengedwenso. Pakati pa galasi wagawo ndi nyali wokwera buluu violet fyuluta. Zimenezi zingakhale FS-1, UFS-3 ndi zina zotero. Yellow fyuluta kupsa pa eyepiece a maso a. Ndi thandizo lawo, chinthu kugwa buluu violet kuwala. Iye anasangalala luminescence. Koma kuwala ungatilepheretse kuwonetsera zowunikira. Choncho, pa njira diso iye n'zometa fyuluta chikasu. Unsembe wa kuyatsa yotentha ndi Koehler analembamo njira, ndi chapadera. Zakulera condenser ayenera kukhala bwinobwino lotseguka. Mu phunziro n'kofunika kugwiritsa ntchito sanali fulorosenti kumiza mafuta. Kuchepetsa umuna nacho anawonjezera thereto nitrobenzene (2-10 akutsikira / 1 g).

Fulorosenti wamamamayikirosikopu: ntchito tizilombo tosaoneka ndi maso

Ubwino wa mtundu uwu wa kufufuza:

1. Kutheka kupeza zithunzi mtundu.
2. Mkulu Mosiyana kudziletsa owala zinthu pa wakuda maziko.
3. Kudziwika ndi kutanthauzira za mitundu ina ya mavairasi ndi majeremusi.
4. Kutheka kuphunzira zinthu chimaonekera ndipo opaque moyo.
5. Kuphunzira moyo njira mu mphamvu zawo.

M'pofunikanso kunena kuti wamamamayikirosikopu fulorosenti amalimbikitsa njira zabwino kwambiri za histology ndi cytochemistry, matenda mwamsanga.